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[Decrease of paraoxonase activity in the first day after abdominal operations in women]
Grzegorz Szymczyk1, Jerzy Bełtowski, Monika Maciejczyk-Pencuła
1I Klinika Ginekologii AM w Lublinie.
Ginekologia Polska
|November 3, 2004
Summary
Post-abdominal surgery in women shows decreased serum paraoxonase (PON1) activity, indicating its role in the body's antioxidant defense. This finding highlights potential implications for patient recovery and oxidative stress management.
Area of Science:
- Biochemistry
- Clinical Medicine
- Surgical Science
Background:
- Paraoxonase (PON1) is a key enzyme in the body's antioxidant defense system, protecting against reactive oxygen species.
- PON1, found in HDL, prevents lipoprotein and plasma membrane peroxidation.
- Reduced PON1 activity is observed in various diseases and after cardiac surgery, but its changes after abdominal operations remain unstudied.
Purpose of the Study:
- To assess the changes in serum PON1 activity in women following abdominal operations.
Main Methods:
- Studied 40 women undergoing abdominal surgeries (hysterectomy, myomectomy, cyst enucleation).
- Collected blood samples pre-operatively and 8 and 24 hours post-operatively.
- Assessed PON1 serum activity spectrophotometrically using paraoxon and phenyl acetate substrates.
Main Results:
- PON1 activity towards paraoxon significantly decreased 8 and 24 hours post-operation (107.6 U/ml and 119.9 U/ml, respectively) compared to pre-operative levels (146.8 U/ml).
- Arylesterase activity (towards phenyl acetate) showed no significant change after surgery.
Conclusions:
- Abdominal operations in women lead to a decrease in serum PON1 activity in the early postoperative period.
- This reduction may be linked to the utilization of PON1 in the antioxidative defense mechanism during surgical stress.