Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sperm surface fibronectin. Expression following capacitation.

F M Fusi1, R A Bronson

  • 1Department of Obstetrics and Gynecology, State University of New York, Stony Brook 11794-8091.

Journal of Andrology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corifollitropin alfa for poor responders patients, a prospective randomized study.

Reproductive biology and endocrinology : RB&E·2020
Same author

Objective evaluation of the viability of cryopreserved oocytes.

Reproductive biomedicine online·2007
Same author

Oocyte cryopreservation: clinical outcome of slow-cooling protocols differing in sucrose concentration.

Reproductive biomedicine online·2007
Same author

Antisperm antibodies: a critical evaluation and clinical guidelines.

Journal of reproductive immunology·2000
Same author

The use of Matrigel at low concentration enhances in vitro blastocyst formation and hatching in a mouse embryo model.

Fertility and sterility·1999
Same author

Progesterone promotes the acrosome reaction in capacitated human spermatozoa as judged by flow cytometry and CD46 staining.

Molecular human reproduction·1999

Fibronectin expression on human sperm increases during capacitation, enhancing sperm-oolemmal adhesion and penetration. Blocking fibronectin with antibodies significantly reduced these essential reproductive processes.

Area of Science:

  • Reproductive Biology
  • Cellular Adhesion
  • Biochemistry

Background:

  • The Arg-Gly-Asp (RGD) sequence is crucial for cell adhesion via integrin receptors.
  • Fibronectin, containing the RGD sequence, is implicated in cell-matrix interactions.
  • Previous studies suggest RGD peptides inhibit sperm-egg interactions.

Purpose of the Study:

  • To investigate fibronectin expression on human spermatozoa during different functional states.
  • To determine the role of fibronectin in sperm-oolemmal adhesion and penetration.

Main Methods:

  • Immunofluorescence using monoclonal and polyclonal anti-fibronectin antibodies.
  • Analysis of fibronectin expression on fresh, capacitated, and acrosome-reacted human spermatozoa.
  • Inhibition studies using anti-fibronectin antibodies in heterologous (human-hamster) and homologous (hamster-hamster) gamete interaction models.

Related Experiment Videos

Main Results:

  • Fresh human spermatozoa showed minimal fibronectin expression (0-16%).
  • Capacitation significantly increased fibronectin expression on spermatozoa (18-100%).
  • Acrosome reaction induction did not alter fibronectin presence or distribution.
  • Anti-fibronectin antibodies significantly reduced sperm-oolemmal adhesion and egg penetration (P < 0.001).

Conclusions:

  • Fibronectin expression on human sperm is upregulated during capacitation.
  • Fibronectin plays a significant physiological role in sperm-oolemmal interactions.
  • Targeting fibronectin may offer strategies for modulating fertility.