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Postoperative Corynebacterium macginleyi endophthalmitis.

Consuelo Ferrer1, José M Ruiz-Moreno, Alejandra Rodríguez

  • 1Departamento de Biología Molecular, VISSUM, Instituto Oftalmológico de Alicante, Alicante, Spain. cferrer@oftalio.com

Journal of Cataract and Refractive Surgery
|November 3, 2004
PubMed
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Polymerase chain reaction (PCR) identified Corynebacterium macginleyi as the cause of chronic endophthalmitis when cultures failed. This molecular technique rapidly diagnosed the infection, leading to successful vancomycin treatment.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Molecular Diagnostics

Background:

  • Chronic endophthalmitis is a serious ocular infection that can occur after cataract surgery.
  • Sterile endophthalmitis is often challenging to diagnose and manage, especially when conventional microbiological methods are negative.

Observation:

  • A 72-year-old male patient presented with chronic endophthalmitis following cataract extraction and a 3-month course of steroid treatment.
  • Ocular samples were subjected to smear, culture, and polymerase chain reaction (PCR) for microbiological analysis.

Findings:

  • PCR amplification of DNA from aqueous samples was positive for bacteria.
  • Sequence analysis of amplified DNA identified Corynebacterium macginleyi as the causative agent within 48 hours.
  • Conventional smear stains and cultures of ocular samples were negative for microbial growth.

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Implications:

  • Polymerase chain reaction and DNA sequencing offer a rapid and sensitive method for diagnosing challenging cases of endophthalmitis, particularly when traditional methods fail.
  • Early and accurate identification of pathogens like Corynebacterium macginleyi is crucial for effective treatment and improved patient outcomes.
  • This case highlights the utility of molecular techniques in identifying microorganisms responsible for chronic ocular infections.