Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic shear stress in parallel-plate flow chambers.

Rommel G Bacabac1, Theo H Smit, Stephen C Cowin

  • 1Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam-Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. rg.bacabac.ocb.acta@med.vu.nl

Journal of Biomechanics
|November 3, 2004
PubMed
Summary

This study characterizes parallel-plate flow chambers for simulating in vivo fluid shear stress. Achieving predictable dynamic shear stress requires specific geometric and frequency conditions for accurate cell response studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perfusion Cell Culture Induces Oxygen Diffusion and Osteogenic Activity in Multi-Channeled Critical-Size Silk Fibroin Scaffolds with Pre-Osteoblasts: Experimental and Finite Element Modeling Study.

Annals of biomedical engineering·2026
Same author

Hypergravity, Microgravity, and Animal Research.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Bone tissue regeneration: role of osteocyte mechanosensing and mechanotransduction.

Stem cells translational medicine·2026
Same author

Influence of cell culture parameters on oxygen diffusion and cell distribution in 3D-porous scaffolds for bone tissue engineering: finite element modeling and experimental verification.

Medical & biological engineering & computing·2026
Same author

Osteogenic differentiation by pre-osteoblasts is enhanced more on 3D-PRINTED poly-ɛ-caprolactone scaffolds coated with collagen and hydroxyapatite than on poly-ɛ-caprolactone/hydroxyapatite composite scaffolds coated with collagen.

Journal of biomaterials applications·2025
Same author

Gravity, microgravity, and artificial gravity: physiological effects, implementation, and applications.

Physiological reviews·2025

Area of Science:

  • Biophysics
  • Cell Biology
  • Fluid Dynamics

Background:

  • Parallel-plate flow chambers are used to simulate in vivo fluid shear stress on cells.
  • Understanding cellular responses to dynamic fluid flow is crucial in physiological environments.
  • Characterization of these chambers for dynamic flow experiments is lacking.

Purpose of the Study:

  • To characterize parallel-plate flow chambers for dynamic fluid flow experiments.
  • To establish conditions for predictable simulation of in vivo fluid shear stress.
  • To enable accurate studies of cellular metabolic responses to shear stress.

Main Methods:

  • Utilized a dimensionless ratio (h / lambda(v)) to determine dynamic wall shear stress magnitude.
  • Introduced a shear factor (T) to scale oscillating components of shear stress.

Related Experiment Videos

  • Defined conditions based on geometric (h / lambda(v)) and frequency (f(0), f(c), m) parameters.

    • Main Results:

    • Identified two critical conditions for predictable dynamic fluid shear stress.
    • Condition 1: h / lambda(v) < 2 (h=plate distance, lambda(v)=viscous penetration depth).
    • Condition 2: f(0) < f(c) / m (f(0)=fundamental frequency, f(c)=critical frequency, m=harmonic mode).

    Conclusions:

    • Established criteria for accurate simulation of dynamic wall shear stress in vitro.
    • Provides a framework for designing experiments using parallel-plate flow chambers.
    • Facilitates reliable investigation of cell behavior under physiological fluid shear stress conditions.