Variation in the dry mass of mouse embryos throughout the preimplantation period

Information on the net balance of embryonic metabolism during the preimplantation period is provided by measurements of embryo dry mass. The dry mass of mouse embryos at 10 defined stages of preimplantation development was measured using the Vickers M86 scanning microinterferometer. The results demonstrate that there is an overall loss of dry mass from the unfertilized ovum (39.10 +/- 0.6 ng) to the late blastocyst stage (32.80 +/- 0.53 ng) with these differences being highly significant. However, there is a significant increase in embryonic dry mass from the 1-cell (36.88 +/- 0.34 ng) to 2-cell stage (40.48 +/- 0.40 ng). These results suggest that protein synthesis exceeds degradation during the 2-cell stage but that from the 4-cell to morula stage the reverse is true. In addition, the variation in dry mass between embryos at the same developmental stage is extremely small, suggesting that this may be a useful indicator of embryonic viability.