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Related Experiment Videos

Resonant mirror biosensor detection method based on yessotoxin-phosphodiesterase interactions.

María-José Pazos1, Amparo Alfonso, Mercedes R Vieytes

  • 1Departamento de Farmacología, Facultad de Veterinaria, USC, 27002 Lugo, Spain.

Analytical Biochemistry
|November 3, 2004
PubMed
Summary

Yessotoxin (YTX) detection in shellfish is now possible using a resonant mirror biosensor. This method quantizes YTX-PDEs interactions, enabling accurate toxin concentration determination in seafood extracts.

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Ecotoxicology and environmental safety·2025

Area of Science:

  • Marine Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Yessotoxin (YTX) is a group of lipophilic marine toxins.
  • Accurate detection methods for YTX are crucial for seafood safety.

Purpose of the Study:

  • To characterize the YTX-PDEs interaction kinetics.
  • To develop a sensitive assay for YTX quantification in shellfish.

Main Methods:

  • Utilized a resonant mirror biosensor with immobilized phosphodiesterase 3',5'-cyclic-nucleotide-specific (PDEs).
  • Measured YTX association kinetics using varying YTX concentrations.
  • Calculated association (k(ass)) and dissociation (k(diss)) rate constants.

Main Results:

  • Determined the association rate constant (k(ass)) as 248+/-40 M(-1)s(-1).

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  • Determined the dissociation rate constant (k(diss)) as 9.36 x 10(-4)+/-1.72 x 10(-4)s(-1).
  • Calculated the kinetic equilibrium dissociation constant (K(D)) for YTX-PDEs as 3.74 x 10(-6)+/-8.25 x 10(-8)M.

    • Conclusions:

    • The YTX-PDEs interaction can be accurately quantified using biosensor technology.
    • The developed assay demonstrates sufficient sensitivity for YTX determination in shellfish extracts.
    • This method offers a viable approach for monitoring YTX contamination in seafood.