Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recurrent Staphylococcus aureus bacteremia.

A I Hartstein1, M E Mulligan, V H Morthland

  • 1Department of Medicine, Oregon Health Sciences University, Portland 97201.

Journal of Clinical Microbiology
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small extracellular particles with big potential for horizontal gene transfer: membrane vesicles and gene transfer agents.

FEMS microbiology letters·2018
Same author

Molecular epidemiology of Candida albicans colonization and fungemia in very low birthweight infants.

The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012
Same author

Mycobacterium abscessus infection in solid organ transplant recipients: report of three cases and review of the literature.

Transplant infectious disease : an official journal of the Transplantation Society·2009
Same author

Imaging techniques used in the diagnosis, staging, and follow-up of patients with myeloma.

Acta radiologica (Stockholm, Sweden : 1987)·2005
Same author

Posterior-inferior glenoid rim shapes by MR imaging.

Surgical and radiologic anatomy : SRA·2005
Same author

Isolated lower extremity metastases, 9 years after initial diagnosis of retinoblastoma.

Skeletal radiology·2005
Same journal

Ebola laboratory preparedness at frontline hospitals: can we or can't we?

Journal of clinical microbiology·2026
Same journal

Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

Journal of clinical microbiology·2026
Same journal

Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

Journal of clinical microbiology·2026
Same journal

Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

Journal of clinical microbiology·2026
Same journal

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same journal

Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

Journal of clinical microbiology·2026
See all related articles

Recurrent Staphylococcus aureus bacteremia can be a relapse or a new infection. Relapsing infections were linked to foreign bodies and vancomycin treatment, while new infections suggest different transmission routes.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Typing

Background:

  • Recurrent Staphylococcus aureus bacteremia poses a significant clinical challenge.
  • Differentiating between relapse and reinfection is crucial for effective treatment strategies.

Purpose of the Study:

  • To differentiate between relapsing and new Staphylococcus aureus bacteremia episodes using molecular typing.
  • To identify factors associated with relapsing Staphylococcus aureus infections.

Main Methods:

  • Restriction Endonuclease Analysis of Plasmid DNA (REAP DNA fingerprinting) and immunoblotting were used to type bacterial isolates.
  • Sequential blood isolates from 10 episodes of recurrent bacteremia in 8 patients were analyzed.
  • Concordance between REAP DNA fingerprinting and immunoblotting was assessed.

Related Experiment Videos

Main Results:

  • Early recurrences (within 2 months) were identical to initial isolates, suggesting relapse.
  • Late recurrences (after 2 months) showed different isolates in 3 of 4 cases, indicating new infections.
  • REAP DNA fingerprinting and immunoblotting showed complete concordance, though 4 isolates required immunoblotting due to lack of plasmid DNA.
  • Bacterial traits remained stable in vivo for up to 3 months.

Conclusions:

  • Molecular typing methods like REAP DNA fingerprinting and immunoblotting are reliable for differentiating Staphylococcus aureus bacteremia recurrence types.
  • Relapsing Staphylococcus aureus bacteremia was associated with intravascular foreign bodies and prior vancomycin therapy.
  • Late recurrences are more likely to represent new infections rather than relapses.