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Developing pineapple fruit has a small transcriptome dominated by metallothionein.

Richard Moyle¹, David J Fairbairn, Jonni Ripi

¹Plant Genetic Engineering Laboratory, Department of Botany, University of Queensland, QLD 4072, Australia.

Journal of Experimental Botany

|November 3, 2004

Summary

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This study analyzed pineapple fruit development by sequencing expressed genes in unripe and ripe tissues. Key differences in gene expression, including those related to fruit development and stress responses, were identified between green and yellow pineapple fruits.

Area of Science:

Plant Molecular Biology
Fruit Development Genomics
Biotechnology

Background:

Understanding pineapple fruit development at the molecular level is crucial for agricultural advancements.
Expressed Sequence Tags (ESTs) provide a snapshot of gene expression in specific tissues and developmental stages.

Purpose of the Study:

To initiate the molecular characterization of pineapple fruit development.
To identify and compare gene expression profiles between unripe green and ripe yellow pineapple fruit.

Main Methods:

Expressed Sequence Tag (EST) sequencing of green unripe and yellow ripe pineapple fruit libraries.
Bioinformatic analysis including sequence clustering, contig assembly, and functional classification.
Northern blot analysis to validate gene expression patterns.

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Main Results:

A highly abundant metallothionein transcript was detected, comprising up to 50% of EST clones.
High redundancy was observed, with a small subset of genes dominating the transcriptome in both fruit stages.
Significant differences in gene expression were found between green and yellow fruit, with abundant genes including fruit bromelain (green) and MADS box genes (yellow).
Both stages showed high expression of genes related to oxidative stress and detoxification.

Conclusions:

Pineapple fruit development involves distinct molecular signatures between unripe and ripe stages.
A limited number of genes play a dominant role in the pineapple transcriptome.
Genes involved in protein synthesis and fruit development, alongside stress response mechanisms, are critical during ripening.