Related Experiment Video
Updated: Aug 21, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in
Ulrich Nübel1, Peter M Schmidt, Edda Reiss
1Robert Koch Institut, 13353 Berlin, Germany. nuebelu@rki.de
Abstract:
We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from seven strains published previously and different from all other bacteria. When 42 oligonucleotide probes targeting polymorphic sites were immobilized on glass slides and hybridized to fluorescently labeled PCR amplification products, one or more mismatches could be discriminated in all but one cases. Hence, hybridization events were highly specific and identification of B. anthracis was straightforward.
Related Concept Videos
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
DNA Microarrays
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Rapid Identification of Pathogens

