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Optimization and validation of a micellar electrokinetic chromatographic method for the analysis of florfenicol

S Hillaert1, W Van den Bossche

  • 1Laboratory of Pharmaceutical Chemistry and Drug Analysis, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium. sandrahillaert@hotmail.com

Journal of Pharmaceutical and Biomedical Analysis
|November 4, 2004
PubMed

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...