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8-oxo-dG elevated in children during leukemia treatment
Deborah D Kennedy1, Regina M Santella, Qiao Wang
1Division of Pediatric Oncology, Department of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA.
Integrative Cancer Therapies
|November 4, 2004
Summary
Oxidative stress markers like 8-oxodeoxyguanosine (8-oxo-dG) change during chemotherapy for childhood acute lymphoblastic leukemia (ALL). Levels decrease initially but rise with more intensive treatment, suggesting 8-oxo-dG
Area of Science:
- Pediatric Oncology
- Biochemistry
- Oxidative Stress Research
Background:
- Oxidative stress changes in children with acute lymphoblastic leukemia (ALL) undergoing chemotherapy are not well-documented.
- 8-oxodeoxyguanosine (8-oxo-dG) is a marker of DNA damage.
- Identifying reliable biomarkers for monitoring treatment response and toxicity is crucial in pediatric oncology.
Purpose of the Study:
- To investigate changes in oxidative stress, specifically 8-oxo-dG levels, in children with ALL during different phases of chemotherapy.
- To assess the potential of 8-oxo-dG as a biomarker for monitoring chemotherapy effects and treatment outcomes in pediatric ALL patients.
- To explore the relationship between antioxidant intake and 8-oxo-dG levels.
Main Methods:
- An observational study involving 103 children diagnosed with ALL.
- Blood samples were collected at diagnosis, interim maintenance (IM), and delayed intensification (DI) phases.
- Immunohistochemical methods were used to measure 8-oxo-dG in blood mononuclear cells and bone marrow; antioxidant intake and plasma levels were also assessed.
Main Results:
- Blood mononuclear cell 8-oxo-dG levels decreased from diagnosis to IM (P = .01) and increased from IM to DI (P < .01).
- Bone marrow 8-oxo-dG levels remained unchanged in a pilot study after 28 days of treatment.
- Higher 8-oxo-dG levels at IM were associated with an increased risk of chemotherapy dose reduction (P = .04).
Conclusions:
- 8-oxodeoxyguanosine (8-oxo-dG) levels in blood mononuclear cells of children with ALL fluctuate during chemotherapy, decreasing initially and increasing during more aggressive phases.
- These findings suggest that 8-oxo-dG may serve as a useful biomarker for monitoring oxidative stress and treatment intensity in pediatric ALL.
- Further research is warranted to fully elucidate the role of 8-oxo-dG and its relationship with antioxidant status and treatment outcomes.