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Updated: Jul 22, 2026

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
Published on: July 29, 2012
New polymorphism of FASN gene in chicken
Graciela Marrube1, Felisa Rozen, Gabriel Bernardo Pinto
1Genetics Area, Animal Production Department, Veterinary School, University of Buenos Aires, Chorroarin 280, 1427, Buenos Aires, Argentina. gmarrube@fvet.uba.ar
Abstract:
Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.
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