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Decrease in calmodulin concentrations during heparin-induced capacitation in bovine spermatozoa.

P Leclerc1, M A Sirard, J G Chafouleas

  • 1Ontogénie et Reproduction, Centre de recherche du CHUL, Ste-Foy, Québec, Canada.

Journal of Reproduction and Fertility
|January 1, 1992
PubMed
Summary
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Heparin reduces bull sperm calmodulin (CaM) concentrations during capacitation, enhancing in-vitro fertilization rates. This CaM reduction involves translocation from sperm to the medium, potentially mediated by sperm proteases.

Area of Science:

  • Reproductive biology
  • Sperm physiology
  • Biochemistry

Background:

  • Sperm capacitation is essential for in-vitro fertilization (IVF).
  • Intracellular calcium and calmodulin (CaM) play critical roles in sperm function.
  • Heparin is a known inducer of sperm capacitation.

Purpose of the Study:

  • To investigate the effect of heparin on intracellular calmodulin concentrations during bull sperm capacitation.
  • To explore the relationship between CaM levels, heparin treatment, and IVF success.
  • To elucidate the mechanism of CaM changes during capacitation.

Main Methods:

  • Radioimmunoassay was used to quantify intracellular calmodulin (CaM) concentrations in bull spermatozoa.
  • Spermatozoa were treated with varying concentrations of heparin to induce capacitation.

Related Experiment Videos

  • CaM levels in the incubation medium were also measured.
  • The effect of the protease inhibitor benzamidine on CaM translocation was assessed.

    • Main Results:

    • Heparin treatment significantly reduced intracellular CaM concentrations in bull spermatozoa in a dose-dependent manner.
    • These reductions in CaM correlated positively with increased in-vitro fertilization rates.
    • CaM concentrations increased in the incubation medium, indicating translocation from the sperm.
    • Benzamidine partially inhibited this CaM translocation, suggesting a role for sperm proteases.

    Conclusions:

    • Heparin-induced sperm capacitation in bulls is associated with a decrease in intracellular calmodulin.
    • CaM appears to translocate from the sperm intracellularly to the surrounding medium during capacitation.
    • Sperm proteases may be involved in the mechanism of CaM translocation.
    • Modulating CaM levels and translocation could be a strategy to improve IVF outcomes.