Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

QA/QC: challenges and pitfalls facing the microarray community and regulatory agencies.

Leming Shi1, Weida Tong, Federico Goodsaid

  • 1US Food and Drug Administration, Center for Toxicoinformatics, Division of Systems Toxicology, National Center for Toxicological Research, HFT-020, 3900 NCTR Road, Jefferson, AR 72079, USA. Leming.Shi@fda.hhs.gov

Expert Review of Molecular Diagnostics
|November 5, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic biomarkers of liver toxicity risk from UK Biobank data.

The pharmacogenomics journal·2025
Same author

Scorpion and centipede alleviates severe asthma through M2 macrophage-derived exosomal miR-30b-5p.

Aging·2022
Same author

Primary mediastinal germ cell tumor and clonally related and unique hematologic neoplasms with i(12p) and TP53 mutation: A report of two cases.

Annals of diagnostic pathology·2022
Same author

Argyrodite-type advanced lithium conductors and transport mechanisms beyond peddle-wheel effect.

Nature communications·2022
Same author

Digital Image Analysis-Based Evaluation of Claudin-1 and Claudin-7 Delocalization in Cutaneous Squamous Cell Carcinoma and in Its Precancerous State.

Journal of oncology·2022
Same author

Evaluation of Serum Proinflammatory Cytokine IL-17A and Tight Junction Protein Claudin-1 in Psoriasis.

Contrast media & molecular imaging·2022

DNA microarray studies face reproducibility and accuracy challenges, hindering personalized medicine. Establishing consensus quality control criteria is crucial for reliable data in drug development and clinical practice.

Area of Science:

  • Genomics
  • Bioinformatics
  • Pharmacology

Background:

  • DNA microarray technology is vital for pharmacogenomic and toxicogenomic research, aiming to advance personalized medicine and drug development.
  • Regulatory agencies like the US FDA are encouraging the use of pharmacogenomic data, but significant challenges impede reliable application.
  • Concerns about reproducibility and accuracy in microarray studies persist, affecting data quality and scientific significance.

Purpose of the Study:

  • To highlight the critical challenges and pitfalls in DNA microarray studies.
  • To emphasize the need for improved data quality, reproducibility, and accuracy.
  • To advocate for collaboration between the scientific community and regulatory agencies to establish quality standards.

Main Methods:

Related Experiment Videos

  • Analysis of published microarray datasets to identify data quality issues.
  • Review of factors affecting microarray study outcomes (technical, instrumental, computational, interpretative).
  • Assessment of intralaboratory data consistency as a foundation for reliable knowledge extraction.

    • Main Results:

    • Profound data quality problems were observed in analyzed datasets.
    • Many laboratories struggle with technical troubleshooting, impacting reliable data generation.
    • Lack of intralaboratory data consistency and cross-laboratory comparisons is a significant issue.

    Conclusions:

    • Establishing consensus quality assurance and control criteria is essential for assessing and ensuring microarray data quality.
    • Identifying critical factors affecting data quality and optimizing/standardizing procedures are necessary.
    • Addressing these fundamental issues is imperative before microarray technology can transition from research to clinical practice.