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Related Experiment Videos

Bub1, a gatekeeper for Cdc20-dependent mitotic exit.

Robert L Margolis1

  • 1Institut de Biologie Structurale J-P. Ebel, 41, rue Jules Horowitz, 38027 Grenoble Cedex 1, France.

Developmental Cell
|November 5, 2004
PubMed
Summary
This summary is machine-generated.

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The mitotic spindle assembly checkpoint ensures cell cycle progression. New findings reveal Bub1 directly phosphorylates Cdc20, inhibiting APC/C activation and arresting cells at metaphase.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The mitotic spindle assembly checkpoint (SAC) is crucial for preventing aneuploidy.
  • SAC arrests cells in metaphase by inhibiting the anaphase-promoting complex/cyclosome (APC/C).
  • Cdc20 is a key activator of APC/C, targeting cyclin B for degradation to allow cell cycle progression.

Purpose of the Study:

  • To elucidate the precise mechanism by which the SAC protein Bub1 suppresses APC/C activity.
  • To identify the direct interaction and regulatory role of Bub1 on Cdc20.

Main Methods:

  • Western blotting
  • Immunoprecipitation
  • In vitro kinase assays
  • Cell-based assays

Related Experiment Videos

Main Results:

  • Bub1 directly phosphorylates Cdc20 at specific sites.
  • Phosphorylation of Cdc20 by Bub1 inhibits its ability to activate the APC/C.
  • This phosphorylation event is critical for maintaining the metaphase arrest mediated by the SAC.

Conclusions:

  • Bub1 acts as a direct inhibitor of Cdc20 through phosphorylation.
  • This mechanism provides a direct link between checkpoint sensing and APC/C regulation.
  • Understanding this pathway is vital for comprehending cell cycle control and potential therapeutic targets.