Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Confocal-line microscopy.

P A Benedetti1, V Evangelista, D Guidarini

  • 1Istituto di Biofisica CNR, Pisa, Italy.

Journal of Microscopy
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of Covid-19 lockdown effects on riverine dissolved organic matter dynamics provides a proof-of-concept for needed regulations of anthropogenic emissions.

The Science of the total environment·2021
Same author

Itchy plaques on trunk and feet in a patient with dark skin: a case of Dystrophic Calcinosis Cutis superimposed on Lichen Simplex Chronicus.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

Roflumilast inhibits leukocyte-platelet interactions and prevents the prothrombotic functions of polymorphonuclear leukocytes and monocytes.

Journal of thrombosis and haemostasis : JTH·2015
Same author

Biophysical processes affecting DOM dynamics at the Arno river mouth (Tyrrhenian Sea).

Biophysical chemistry·2014
Same author

Glycogen synthase kinase-3 negatively regulates tissue factor expression in monocytes interacting with activated platelets.

Journal of thrombosis and haemostasis : JTH·2011
Same author

Thermoelectric photocathode cooler allowing rapid interchange of end-on photomultipliers.

Applied optics·2010

The novel confocal-line (CL) microscopy technique uses line illumination and linear detection, offering a flexible alternative to confocal-point (CP) methods for diverse imaging applications. This approach enables simultaneous multi-modal imaging and spectral analysis without moving optical parts.

Area of Science:

  • Microscopy and Imaging Technologies
  • Optical Physics
  • Biomedical Engineering

Background:

  • Confocal microscopy is a powerful imaging technique, but conventional confocal-point (CP) systems can be limited by scanning speed and mechanical complexity.
  • There is a need for advanced microscopy techniques that offer enhanced flexibility, speed, and multi-modal capabilities for various research applications.

Purpose of the Study:

  • To introduce and evaluate the confocal-line (CL) technique as a novel alternative to traditional confocal-point (CP) microscopy.
  • To demonstrate the adaptability and performance of CL microscopy for biological and non-biological imaging tasks.
  • To explore extensions of CL microscopy for advanced applications such as spectral imaging.

Main Methods:

  • The confocal-line (CL) technique was developed, utilizing line-shaped illumination and linear image detection.

Related Experiment Videos

  • Prototype instrumentation was constructed based on CL principles.
  • The system was tested on selected applications, including simultaneous collection of transmission, reflection, and emission images.
  • Modifications involving a slit, polychromator, and area detector were implemented for spectral imaging capabilities.

    • Main Results:

    • The CL technique demonstrated performance and features suitable for a variety of applications, functioning as a one-dimensional confocal system.
    • The absence of moving components in the optical path and the use of electronic linear imagers allowed for flexible and fast operation.
    • Simultaneous collection of transmission, reflection, and emission images from the same specimen area was achieved with minor optical adjustments.
    • Successful extensions to spectral imaging were demonstrated through specific optical modifications.

    Conclusions:

    • The confocal-line (CL) technique offers a viable and advantageous alternative to confocal-point (CP) microscopy, particularly for applications requiring speed and flexibility.
    • CL microscopy facilitates efficient multi-modal imaging and spectral analysis, broadening its utility in basic and applied research.
    • The developed prototype instrumentation validates the principles of CL microscopy and its potential for diverse scientific investigations.