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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Preparation and characterization of long methacrylate monolithic column for capillary liquid chromatography
Xin Shu1, Lingxin Chen, Bingcheng Yang
1Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Abstract:
Long methacrylate monolithic columns (100 cm x 320 microm i.d.) were prepared from silanized fused-silica capillaries of 320 microm i.d. by in situ copolymerization of butyl methacrylate (BMA) with ethylene dimethacrylate (EDMA) in the presence of a suitable porogen. The separation performance and selectivity of the column were evaluated and compared with a 25 cm x 320 microm i.d. column prepared in the same way by capillary high-performance liquid chromatography (micro-HPLC). The results showed that the 1 m long monolithic column can generate 33 x 10(3) plate number and exhibited good permeability, higher sample loadability, and separation capability.
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