Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Graft engineering for allogeneic haploidentical stem cell transplantation.

Antonio Tabilio1, Franca Falzetti, Tiziana Zei

  • 1Department of Clinical and Experimental Medicine, Hematology and Clinical Immunology, University of Perugia, Perugia, Italy. atabilio@unipg.it

Blood Cells, Molecules & Diseases
|November 6, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regional epidemiologic data call for enhanced attention to Clostridioides difficile infections in Italy: Results from a population-based study.

Journal of infection and public health·2025
Same author

Clostridioides difficile meets the adenosine system: the art of manipulating host homeostasis.

Journal of biomedical science·2025
Same author

Bcor loss promotes Richter transformation of chronic lymphocytic leukemia associated with Notch1 activation in mice.

Leukemia·2025
Same author

Nanotechnology Advances in the Detection and Treatment of Lymphoid Malignancies.

International journal of molecular sciences·2024
Same author

PhosphoLipidome Alteration Induced by <i>Clostridioides difficile</i> Toxin B in Enteric Glial Cells.

Cells·2024
Same author

Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML.

Nature communications·2024

Haploidentical stem cell transplants offer a vital option for patients lacking matched donors. Optimized graft processing ensures high stem cell doses and minimal lymphocytes, improving engraftment and reducing complications like graft-versus-host disease.

Area of Science:

  • Hematopoietic Stem Cell Transplantation
  • Immunology
  • Oncology

Background:

  • Haploidentical stem cell transplantation (HSCT) has emerged as a crucial therapeutic option for hematological malignancies, expanding transplant eligibility to approximately 50% of patients lacking matched donors.
  • Optimal graft preparation is paramount for successful HSCT, necessitating specific criteria for hematopoietic stem cells, T-lymphocytes, and B-lymphocytes to mitigate risks.
  • The evolution of graft processing techniques over the past decade has focused on achieving these critical requirements for haploidentical transplants.

Purpose of the Study:

  • To analyze the evolution of graft processing procedures for haploidentical stem cell transplantation over the last ten years.
  • To identify the essential requisites for graft preparation in haploidentical HSCT, including megadose hematopoietic stem cells and minimal T- and B-lymphocytes.

Related Experiment Videos

  • To evaluate the efficacy of current graft processing technologies in meeting these requirements and improving transplant outcomes.

    • Main Methods:

    • Review of historical graft processing methods, including E-rosetting and soybean agglutination.
    • Analysis of sequential advancements in negative and positive selection techniques for hematopoietic stem cells.
    • Evaluation of the current one-step positive selection method for graft processing in haploidentical HSCT.

    Main Results:

    • Current positive selection methods achieve 70-80% hematopoietic stem cell recovery, ensuring megadose targets in over 70% of cases.
    • Effective depletion of T-lymphocytes (>4 logs) and B-lymphocytes (>3 logs) is consistently achieved, significantly reducing risks of GvHD and EBV-related disorders.
    • High primary, sustained engraftment rates (>90%) and reduced incidence of severe acute GvHD and EBV-PTLD have been observed.

    Conclusions:

    • Modern graft processing technologies, particularly one-step positive selection, reliably meet the stringent requirements for haploidentical HSCT.
    • Advancements in graft preparation have significantly improved transplant outcomes, including engraftment rates and reduced GvHD and EBV-related complications.
    • Automated, time-saving graft processing devices enhance reproducibility, reliability, and safety, facilitating the widespread clinical application of haploidentical transplantation.