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Inwardly rectifying chloride channel activity in intestinal pacemaker cells.

Yaohui Zhu¹, Andrea Mucci, Jan D Huizinga

¹McMaster Univ., HSC-3N5C, 1200 Main St. West, Hamilton, ON L8N 3Z5, Canada.

American Journal of Physiology. Gastrointestinal and Liver Physiology

|November 6, 2004

Summary

Interstitial cells of Cajal (ICC) possess an inwardly rectifying chloride current crucial for gut pacemaker activity. This chloride channel

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Area of Science:

Physiology
Gastroenterology
Ion Channel Biology

Background:

Chloride channels are implicated in gut pacemaker activity.
The specific characteristics of chloride channels in interstitial cells of Cajal (ICC) remain largely uncharacterized.

Purpose of the Study:

To identify and characterize whole-cell chloride currents in ICC.
To investigate the inward rectification properties of these currents.

Main Methods:

Whole-cell patch-clamp electrophysiology was employed on ICC.
Single-channel activity was analyzed using ramp protocols.
The effects of extracellular cation composition, DIDS, and 9-anthracene carboxylic acid were assessed.

Main Results:

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An inwardly rectifying chloride current was identified in ICC.
This current was insensitive to changes in extracellular cation concentration.
The current followed the chloride equilibrium potential and was inhibited by DIDS and 9-anthracene carboxylic acid.
Inward rectification was attributed to reduced single-channel open probability, not conductance.
Evidence suggests cooperation between 30-pS channels, varying with membrane potential.

Conclusions:

An inwardly rectifying chloride channel plays a significant role in gut pacemaker activity.
Understanding these channels in ICC is key to comprehending gut motility regulation.