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Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

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Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
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SNAREs and Membrane Fusion01:43

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Updated: Jul 6, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

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Published on: October 19, 2012

Tracking SNARE complex formation in live endocrine cells.

Seong J An1, Wolfhard Almers

  • 1Vollum Institute L-474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97201, USA.

Science (New York, N.Y.)
|November 6, 2004
PubMed
Summary
This summary is machine-generated.

Researchers studied SNARE protein complex formation in neurons. They found a syntaxin-SNAP25 complex that may be a precursor in the exocytosis process, assembling reversibly with calcium influx.

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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • SNARE proteins, including syntaxin, SNAP25, and synaptobrevin, are crucial for neuronal exocytosis.
  • The precise steps involved in SNARE core complex formation remain largely unknown.
  • Neuronal exocytosis is a fundamental process for neurotransmitter release.

Purpose of the Study:

  • To investigate the in vivo steps of SNARE complex formation.
  • To identify potential precursor complexes in the exocytosis pathway.
  • To understand the role of calcium in SNARE complex assembly.

Main Methods:

  • Utilized a fluorescently tagged version of SNAP25 to monitor complex formation in real-time.
  • Employed PC12 cells as a model system for studying neuronal exocytosis.
  • Investigated the impact of specific mutations and calcium influx on complex assembly.

Main Results:

  • Evidence for a high-affinity syntaxin-SNAP25 complex was observed in vivo.
  • This complex formation required only the amino-terminal SNARE motif of SNAP25.
  • The syntaxin-SNAP25 complex assembled reversibly upon calcium entry during depolarization.

Conclusions:

  • The identified syntaxin-SNAP25 complex may represent a crucial precursor to the SNARE core complex.
  • This precursor complex likely participates in a calcium-dependent priming step of exocytosis.
  • Understanding these early steps provides insights into the regulation of neurotransmitter release.