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Updated: Aug 21, 2026

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
Published on: October 26, 2019
Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms
Stefan Leuko1, Andrea Legat, Sergiu Fendrihan
1Cyanobacteria and Astrobiology Research Laboratory, University of New South Wales, Sydney, Australia.
Abstract:
Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.
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