Related Experiment Video
Updated: Aug 21, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria
Maryvonne Arnaud1, Arnaud Chastanet, Michel Débarbouillé
1Unité de Biologie des Bactéries Pathogènes à Gram Positif, Institut Pasteur, Paris, France.
Abstract:
A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.
More Related Videos
Related Concept Videos
Bacterial Transformation
Transformation
Transduction

