Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasminogen activator expression correlates with genetic differences in vascular remodeling.

Vyacheslav A Korshunov1, Marina A Solomatina, Olga S Plekhanova

  • 1Center for Cardiovascular Research, Aab Institute of Biomedical Sciences and Department of Medicine, University of Rochester, 601 Elmwood Ave, Rochester, NY 14642, USA.

Journal of Vascular Research
|November 6, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endothelial-to-mesenchymal transition (EndMT) in atherosclerosis: mechanisms, models and therapies.

Cardiovascular research·2026
Same author

Special Issue "Fundamental and Practical Perspectives in Regenerative Medicine: Proceedings of the VI National Congress of Regenerative Medicine (2024)".

International journal of molecular sciences·2026
Same author

Endothelial cell RpL17-dependent translational control mediates intima-media thickening in response to disturbed flow.

bioRxiv : the preprint server for biology·2026
Same author

Corrigendum to "Impaired spine formation and learning in GPCR kinase 2 interacting protein-1 (GIT1) knockout mice" [Brain Res. 1317 (2010) 218-26].

Brain research·2026
Same author

Immortalization of Cultured Cells in Regenerative Biomedicine: Approaches, Opportunities, and Limitations.

Biochemistry. Biokhimiia·2025
Same author

Overexpression of BDNF and uPA Combined with the Suppression of Von Hippel-Lindau Tumor Suppressor Enhances the Neuroprotective Activity of the Secretome of Human Mesenchymal Stromal Cells in the Model of Intracerebral Hemorrhage.

International journal of molecular sciences·2025

Vascular remodeling in mouse carotid arteries shows genetic differences in intima-media thickening (IMT). Increased tissue plasminogen activator (t-PA) and urokinase plasminogen activator (u-PA) expression correlated with IMT severity.

Area of Science:

  • Cardiovascular Biology
  • Vascular Biology
  • Genetics

Background:

  • Intima-media thickening (IMT) of the carotid artery is a vascular remodeling process linked to coronary artery disease risk.
  • Vascular remodeling involves complex cell-matrix interactions influenced by blood flow.
  • Understanding the genetic basis of flow-induced vascular remodeling is crucial.

Purpose of the Study:

  • To investigate the role of plasminogen activators (PAs) and matrix metalloproteinases (MMPs) in flow-induced carotid artery remodeling.
  • To compare the genetic determinants of vascular remodeling in response to altered blood flow between two inbred mouse strains.
  • To evaluate the correlation between specific matrix-degrading enzyme expression and the extent of IMT.

Main Methods:

  • Partial carotid ligation was performed in C57Bl/6J and FVB/NJ mice to create low (LCA) and high (RCA) flow conditions.

Related Experiment Videos

  • Expression levels of urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA), MMP-2, and MMP-9 were measured in ligated carotids.
  • Intima-media thickening (IMT) volume was quantified and compared between mouse strains and flow conditions.

    • Main Results:

    • Greater vascular remodeling (IMT) occurred in response to low carotid artery flow (LCA) compared to high flow (RCA).
    • Maximal IMT volume was significantly greater in FVB mice than in C57 mice, despite similar flow reduction.
    • Increased expression of t-PA and u-PA strongly correlated with increased IMT, while MMP-2, MMP-9, and TIMP-2 did not differ between strains.

    Conclusions:

    • Flow-induced intima-media thickening of the carotid artery is a genetically determined process.
    • The expression of t-PA and u-PA are key factors correlating with the extent of carotid artery remodeling in response to altered blood flow.
    • These findings highlight genetic variations in matrix-degrading enzyme expression contributing to differential vascular remodeling responses.