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Related Experiment Videos

Perp is required for tissue-specific cell survival during zebrafish development.

M Nowak1, C Köster, M Hammerschmidt

  • 1Max-Planck Institute for Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany.

Cell Death and Differentiation
|November 6, 2004
PubMed
Summary

The tumor suppressor p53 protein influences cell fate. A gene called perp, normally pro-apoptotic, acts anti-apoptotic during zebrafish development, regulating cell survival in specific tissues.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • The tumor suppressor p53 protein mediates cell cycle arrest or apoptosis via transcriptional targets.
  • The transmembrane protein Perp is a p53 target gene upregulated in apoptotic cells, but its developmental role is unclear.

Purpose of the Study:

  • To isolate and characterize the zebrafish perp homolog.
  • To investigate the role of Perp during normal zebrafish development and in response to cellular stress.

Main Methods:

  • Isolation and characterization of zebrafish perp.
  • Overexpression studies in zebrafish embryos.
  • UV-induced cell death assays.
  • Antisense-mediated knockdown of Perp function.

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Main Results:

  • Zebrafish Perp overexpression induces apoptosis in early embryos.
  • Perp contributes to p53-dependent and UV-induced cell death.
  • Perp exhibits p53-independent, tissue-specific expression during normal development.
  • Loss of Perp function increases apoptosis in developing skin and notochord cells.

Conclusions:

  • Perp has a dual role: pro-apoptotic in stressed cells and anti-apoptotic in normal development.
  • Perp regulates tissue-specific cell survival during zebrafish development.
  • The findings reveal a novel anti-apoptotic function for Perp in development.