Related Experiment Video
Updated: Aug 8, 2026
Intravitreous Injection for Establishing Ocular Diseases Model
Published on: October 1, 2007
[Experimental intraocular introduction of blood serum of patients with endophthalmitis]
Abstract:
Effects of blood serum from patients with endophthalmitis, who systematically received gentamicin at therapeutic dose, exerted on the retina of healthy rabbits were experimentally studied. A moderate toxic effect produced by gentamicin on the retina, when it was administered in the vitreous body of rabbits, was detected yet on day 1 of the experiment. Changes of retinal electrogenesis were aggravating and, beginning from day 7, there were noted profound changes of the functional activity in all retinal layers. According to the results, the blood serum from patients with endophthalmitis, who were systematically receiving gantamicin at therapeutic dose, does not virtually have any negative effect on the functional condition of the retina and can be used for intracavitary administration.
Insights
Blood serum from endophthalmitis patients receiving gentamicin showed no negative retinal effects in rabbits. This suggests the serum is safe for intracavitary administration, despite gentamicin
Area of Science:
- Ophthalmology
- Toxicology
- Pharmacology
Background:
- Endophthalmitis requires treatment, often with gentamicin.
- Gentamicin can have toxic effects on the retina when administered directly into the eye.
- Systemic gentamicin administration in patients may alter serum composition.
Purpose of the Study:
- To investigate the toxic effects of blood serum from patients treated with systemic gentamicin on rabbit retinas.
- To determine the safety of using this serum for intracavitary administration.
Main Methods:
- Experimental study on healthy rabbits.
- Administration of blood serum from endophthalmitis patients receiving systemic gentamicin.
- Monitoring of retinal electrogenesis and functional activity.
Main Results:
- Gentamicin administered directly into the vitreous body of rabbits caused moderate retinal toxicity from day 1.
- Progressive aggravation of retinal electrogenesis changes and profound functional activity alterations were observed from day 7.
- Blood serum from patients receiving systemic gentamicin showed no significant negative impact on retinal function in rabbits.
Conclusions:
- Blood serum from endophthalmitis patients on systemic gentamicin therapy is virtually non-toxic to the retina.
- This serum is potentially safe for intracavitary administration in ophthalmic treatments.
