[Description and value of the Microvision system for pollen analysis]

Currently, two techniques of analysis are classically used by the aerobiological networks: the technique of the 12 vertical lines allow to follow the evolution by bi-hourly periods of pollination, but the segmentation of the sample doesn't allow to visualise pollen clouds of short duration; the technique of 2 or 3 horizontal lines allow to follow the daily evolution, but only the daily totals are recorded. The MICROVISION system adapted by the RNSA to the pollen analysis allow to keep the horizontal method, which is faster and continue, and to record automatically in the database the hourly or bi-hourly data. So each pollen is recorded by its coordinates with a precision of 1 micron (1.8 seconds). First, we realised a cartography of the impaction of the pollen grains on the strip, in order to locate the more representative horizontal lines. Secondly, the coupling of the microscope with the Microvision system and the software of data acquiring elaborated by the RNSA allow to propose a more reliable and faster procedure for pollen analysis.