GEC1, a protein related to GABARAP, interacts with tubulin and GABA(A) receptor
Virginie Mansuy1, Wilfrid Boireau, Annick Fraichard
1Equipe Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, Université de Franche-Comté, U.F.R. Sciences et Techniques, IFR 133, 16 route de Gray, 25030 Besançon Cedex, France.
Abstract:
We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA(A) receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA(A) receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA(A) receptor.
More Related Videos
Related Concept Videos
Activation and Inactivation of G Proteins
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
GTPases and their Regulation
Large G-proteins, also known...
GTPases and their Regulation
Large G-proteins, also known...
G-protein Coupled Receptors
G-protein Coupled Receptors


