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Cytochemical location of urease in the cell wall of two different lichen phycobionts
A M Millanes1, B Fontaniella, M L García
1Laboratory of Plant Biology, Faculty of Biology, Complutense University, C/José Antonio Novias s/n, 28040 Madrid, Spain.
Abstract:
The enzyme urease has been located in the cell wall of recently isolated phycobionts from Evernia prunastri and Xanthoria parietina lichens. Cytochemical detection is achieved by producing a black, electron-dense precipitate of cobalt sulfide proceeding from CO(2) evolved from urea in the presence of cobalt chloride. Cellular fractionation reveals that about 80% of total urease activity was associated to the cell wall on both phycobionts whereas only 20% was recovered as soluble protein.
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