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Updated: Aug 21, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
DNA-modified carbon paste electrode applied to the study of interaction between rifampicin (RIF) and DNA in solution
S Th Girousi1, I Ch Gherghi, M K Karava
1Analytical Chemistry Laboratory, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. girousi@chem.auth.gr
Abstract:
Rifampicin (RIF) is an antibiotic widely used against tuberculosis and a DNA intercalator. The interaction of RIF with double-stranded (ds) and single-stranded (ss) calf thymus DNA was studied in solution as well as at the electrode surface by means of transfer voltammetry using differential pulse as stripping mode with carbon paste electrode (CPE) at 0.2M acetate buffer at pH 5.0 and at 0.2M phosphate buffer pH 7.4. Differentiations in the above-mentioned interaction at different pH values are presented and compared in order to optimize the detection of Rifampicin (RIF).

