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Updated: Aug 21, 2026

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Determination and in-process control of zolpidem synthesis by high-performance liquid chromatography
L Laviana1, C Mangas, F Fernández-Marí
1Department of Physical and Analytical Chemistry, University of Oviedo, Química Física y Analítica, Julian Clavería, Oviedo, Spain.
Abstract:
A high-performance liquid chromatographic assay with diode-array detection has been developed for the in-process control of zolpidem synthesis and for the analysis of the drug and its synthetic intermediates. The separation uses a 4.6mm i.d. reversed-phase Kromasil C(18) (150 mm) column, 5 microm particle size with a gradient elution mode of acetonitrile and 0.02 M NH(4)OAc (adjusted to pH 8.0) as the mobile phase (flow rate 1.0 mlmin(-1)). The analysis is performed in 12 min. The method is simple, rapid and highly specific.
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High-Performance Liquid Chromatography: Instrumentation
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High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process: