Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray gene expression profiling during the segmentation phase of zebrafish development.

Elwood Linney1, Betsy Dobbs-McAuliffe, Hedieh Sajadi

  • 1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
|November 10, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The transcriptional response to oxidative stress during vertebrate development: effects of tert-butylhydroquinone and 2,3,7,8-tetrachlorodibenzo-p-dioxin.

PloS one·2014
Same author

Brief embryonic strychnine exposure in zebrafish causes long-term adult behavioral impairment with indications of embryonic synaptic changes.

Neurotoxicology and teratology·2012
Same author

Identification and characterization of a functional zebrafish smrt corepressor (ncor2).

Gene·2011
Same author

The negative side of retinoic acid receptors.

Neurotoxicology and teratology·2011
Same author

Critical duration of exposure for developmental chlorpyrifos-induced neurobehavioral toxicity.

Neurotoxicology and teratology·2011
Same author

Persistent behavioral impairment caused by embryonic methylphenidate exposure in zebrafish.

Neurotoxicology and teratology·2011
Same journal

Review: Reproductive toxicity of benzo[α]pyrene: Effects, mechanisms, and perspectives.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same journal

Acute sublethal ammonia exposure suppresses neurotransmitter expression and impairs behaviors in the early development stages of zebrafish.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same journal

Ecological risks of olivine with different particle sizes and dosages on planktonic ecosystems during ocean alkalinization.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same journal

Integrative QSAR modelling of multi-species pesticide baseline (narcosis) toxicity.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same journal

Elephant alcohol dehydrogenase and aldehyde dehydrogenase: Genomic, transcriptomic, and enzymatic analyses raise questions about anecdotal inebriation.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same journal

Effects of long-term exposure to 2,6-di-tert-butylhydroxytoluene and its transformation product 2,6-di-tert-butylbenzoquinone on development and reproduction in zebrafish.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
See all related articles

This study tracked gene expression changes in zebrafish embryos over 24 hours. Key genes involved in development and protein synthesis showed significant expression shifts during this critical period.

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Understanding early embryonic gene regulation is crucial for developmental biology.
  • Zebrafish (Danio rerio) embryos provide a powerful model for studying rapid developmental processes.

Purpose of the Study:

  • To comprehensively analyze gene expression patterns during the first 24 hours of zebrafish embryonic development.
  • To identify genes with dynamic expression changes and specific regulatory patterns.

Main Methods:

  • Utilized a long oligonucleotide microarray with over 16,000 probes to analyze 15,512 unique transcripts.
  • Isolated total RNA from staged zebrafish embryos at 2-hour intervals over a 24-hour period.
  • Quantified gene expression levels to identify up-regulated and down-regulated transcripts.

Related Experiment Videos

Main Results:

  • Approximately 27% of the probe set detected transcripts at any given time point.
  • Nearly 4000 genes exhibited altered expression during the first 24 hours postfertilization (hpf).
  • Significant numbers of genes were up- and down-regulated by 12 hpf and 24 hpf, with notable phasic regulation in five genes.

Conclusions:

  • Identified specific genes like germ cell nuclear factor and mesogenin with transient expression during gastrulation.
  • Observed up-regulation of ribosomal proteins at 12 hpf, suggesting active protein synthesis.
  • Detected late up-regulation of muscle-specific genes, correlating with somite development and differentiation.