gsh1 demarcates hypothalamus and intermediate spinal cord in zebrafish

We have cloned and characterized the expression of zebrafish genetic screen homeobox 1 (gsh1). Early expression is confined to hindbrain rhombomeres; by mid-somitogenesis gsh1 is expressed in precise domains within the mesencephalon and diencephalon, as well as in intermediate spinal cord. Double-label experiments revealed that the diencephalic domain is coincident with hypothalamus and that spinal cord expression is in a region that generates interneurons. These data suggest gsh1 may play a role in patterning cell types generated in these domains.