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Published on: July 18, 2017
Inhibition of transfected PTEN on human colon cancer
Shou-Shui Xu1, Wen-Lu Shen, Song-Ying Ouyang
1Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, Guangdong Province, China. smile4612003@yahoo.com.cn
Aim:
To study the inhibitory effect of transfected PTEN on LoVo cells.
Methods:
Human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Before or after transfection, the effects of 5-Fu on inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT.
Results:
PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cells.
Conclusion:
Transfected PTEN can remarkably up-regulate PTEN expression in LoVo cells and promote the apoptosis. PTEN transfection is associated with 5-Fu treatment effect and has a cooperatively cytotoxic effect.
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