Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detecting ATP release by a biosensor method.

Seiji Hayashi1, Akihiro Hazama, Amal K Dutta

  • 1Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan.

Science'S STKE : Signal Transduction Knowledge Environment
|November 13, 2004
PubMed
Summary

This study introduces a novel biosensor for measuring local adenosine 5'-triphosphate (ATP) near cell surfaces. This method overcomes limitations of bulk assays by detecting real-time ATP signaling crucial for cell communication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Influential factors of long-term and seasonal <sup>137</sup>Cs change in agricultural and forested rivers: Temperature, water quality and an intense Typhoon Event" [Environ. Pollut. 338, 122617].

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Association between postnatal anthropometric growth by term and high-performing neurodevelopment at age 3 years in extremely preterm infants.

Journal of perinatology : official journal of the California Perinatal Association·2026
Same author

Corrigendum to "Response of <sup>137</sup>Cs dynamics in dam lakes to temperature and weather conditions" [Environ. Pollut. 378, 126445].

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Linking forest compartments to the long-term decline rates of <sup>137</sup>Cs in stream fish: A contaminated headwater catchment in Fukushima.

Journal of environmental radioactivity·2026
Same author

Effect of VSOR/VRAC blocker tamoxifen on contractile activity of primary neonatal cardiomyocytes and isolated perfused heart.

BMC research notes·2026
Same author

Wildfires Oppositely Modify Radiocesium Mobility in Forest Litter and Soils: Evidence from Radiologically Contaminated Areas in Fukushima.

Environmental science & technology·2026

Area of Science:

  • Cell biology
  • Neuroscience
  • Biochemistry

Background:

  • Extracellular adenosine 5'-triphosphate (ATP) is vital for intercellular signaling.
  • Conventional assays often misrepresent local ATP concentrations due to degradation and diffusion barriers.
  • Understanding cell surface ATP dynamics is critical for various physiological processes.

Purpose of the Study:

  • To develop a biosensor for real-time measurement of local ATP concentration at the cell surface.
  • To overcome the limitations of bulk ATP measurements.
  • To investigate cell-to-cell signaling mediated by extracellular ATP.

Main Methods:

  • Utilized purinergic P2X receptors expressed in PC12 or HEK293 cells as biosensors.
  • Employed the patch-clamp technique to measure P2X-mediated currents.

Related Experiment Videos

  • Calibrated ATP release using controlled local ATP applications.

    • Main Results:

    • Successfully measured local ATP concentrations on the cell surface in real time.
    • Demonstrated the feasibility of using P2X receptor-expressing cells as biosensors.
    • Quantified ATP release from specific sites on target cells.

    Conclusions:

    • The developed biosensor provides a sensitive method for assessing local extracellular ATP.
    • This technique offers new insights into ATP-mediated cell communication.
    • The biosensor can be applied to study ATP release in various biological contexts.