Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chronic prostatitis: Charlottesville to Seattle.

John N Krieger1, Donald E Riley

  • 1Department of Urology, University of Washington School of Medicine, Seattle, Washington, USA.

The Journal of Urology
|November 13, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A large-scale multi-ancestry genome-wide association study of chronic prostatitis/chronic pelvic pain syndrome in men.

Nature communications·2026
Same author

Urologic chronic pelvic pain syndrome 3-year symptom trajectories: the Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Symptom Patterns Study.

BJU international·2025
Same author

Widespread pain phenotypes impact treatment efficacy results in randomized clinical trials for interstitial cystitis/bladder pain syndrome: a Multidisciplinary Approach to the Study of Chronic Pelvic Pain network study.

Pain·2024
Same author

Neonatal Male Circumcision: Clearly Beneficial for Public Health or an Ethical Dilemma? A Systematic Review.

Cureus·2024
Same author

Neurobiology and long-term impact of bladder-filling pain in humans: a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) research network study.

Pain·2023
Same author

Infant Circumcision for Sexually Transmitted Infection Risk Reduction Globally.

Global health, science and practice·2022

Sensitive molecular methods detected fastidious microorganisms in men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). These findings may improve diagnosis and treatment for this condition.

Area of Science:

  • Urology
  • Microbiology
  • Molecular Diagnostics

Background:

  • Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) affects many men.
  • Traditional bacterial cultures often fail to identify causative agents in CP/CPPS.
  • Sensitive molecular techniques are needed to detect fastidious microorganisms.

Purpose of the Study:

  • To investigate the presence of fastidious microorganisms in men with CP/CPPS using molecular methods.
  • To determine if specific pathogens or microbial DNA are associated with CP/CPPS.
  • To explore potential links between microbial findings and inflammation markers.

Main Methods:

  • Evaluated 135 men with CP/CPPS using clinical assessment and expressed prostatic secretions analysis.
  • Employed specific and broad-spectrum polymerase chain reaction (PCR) assays on prostate tissue.

Related Experiment Videos

  • Excluded patients with other urogenital infections to ensure focus on CP/CPPS.

    • Main Results:

    • Specific PCR identified Mycoplasma genitalium, Chlamydia trachomatis, or Trichomonas vaginalis in 8% of subjects.
    • Broad-spectrum PCR detected tetracycline resistance DNA in 25% and 16S rDNAs in 77% of subjects.
    • Higher white blood cell counts in prostatic secretions correlated with 16S rDNA detection in prostate tissue (p <0.01).

    Conclusions:

    • Fastidious microorganisms like C. trachomatis, T. vaginalis, and M. genitalium may be implicated in some CP/CPPS cases.
    • The presence of microbial DNA, including antibiotic resistance genes, is common in CP/CPPS patients.
    • Further research into the role of these organisms can refine diagnostic and treatment strategies for CP/CPPS.