Potentiometric immunosensor using artificial antibody based on molecularly imprinted polymers

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Development of sensitive and specific biosensors is crucial for clinical diagnostics.
• Potentiometric sensors offer a simple transduction mechanism for analyte detection.
• Molecularly imprinted polymers (MIPs) provide a robust platform for creating artificial receptors.

Purpose of the Study:

• To develop a potentiometric artificial immunosensor based on MIPs for easy clinical analysis.
• To evaluate the sensor's specificity and response characteristics using serotonin as a model analyte.
• To explore the potential for creating multimicrosensor arrays for diverse analytes.

Main Methods:

• Fabrication of a potentiometric immunosensor using molecularly imprinted polymers.
• Evaluation of sensor response to serotonin and structurally similar compounds (tryptamine, acetaminophen, procainamide).
• Determination of sensor response time, determination limit, and detection limit.

Main Results:

• The sensor demonstrated high responsiveness to serotonin, confirming specific recognition.
• The sensor showed minimal response to tryptamine, acetaminophen, and procainamide, indicating high selectivity.
• Rapid response time of approximately 12 seconds was achieved.
• Determination and detection limits were found to be 1 µmol/L and 100 pmol/L, respectively.

Conclusions:

• The developed potentiometric artificial immunosensor exhibits high specificity and sensitivity for serotonin detection.
• The simple design and rapid response time make it suitable for easy clinical analysis.
• The strategy is applicable for constructing arrays of artificial immunosensors for various analytes.