Solid phase extraction on sax columns as an alternative for ochratoxin A analysis in maize
J M Pelegri1, C Velázquez, V Sanchís
1Chemistry Department, Universitat de Lleida, Lleida, España.
Revista Iberoamericana De Micologia
|November 13, 2004
Abstract:
A sensitive and reliable method is described for the determination of ochratoxin A (OTA) in maize. An extraction and clean-up procedure was used, with chloroform-phosphoric acid as the extractant, and liquid-liquid partition and anion-exchange chromatography (SAX columns) for the clean-up. Quantification of toxin is achieved by high performance liquid chromatography (HPLC). Recoveries were between 81-94 % at 3-90 ng/g levels. The detection limit was 0.02 ng.
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