Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

DNA-psoralen interaction: a single molecule experiment.

M S Rocha¹, N B Viana, O N Mesquita

¹Departamento de Física, ICEX, Universidade Federal de Minas Gerais, Caixa Postal 702, Belo Horizonte, CEP 30123-970 Minas Gerais, Brazil.

The Journal of Chemical Physics

|November 13, 2004

Summary

Researchers studied DNA elasticity using optical tweezers and found that psoralen, a light-sensitive drug, alters DNA

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Imidazolium-based ionic liquids binding to DNA: Mechanical effects and thermodynamics of the interactions.

International journal of biological macromolecules·2022

Same author

Caffeine modulates the intercalation of drugs on DNA: A study at the single molecule level.

Biophysical chemistry·2021

Same author

Olfactory response of <i>Trichogramma pretiosum</i> (Hymenoptera: Trichogrammatidae) to volatiles induced by transgenic maize.

Bulletin of entomological research·2021

Same author

The predatory mite Neoseiulus californicus (Acari: Phytoseiidae) does not respond for volatiles of maize infested by Tetranychus urticae (Acari: Tetranychidae).

Brazilian journal of biology = Revista brasleira de biologia·2021

Same author

Bessel beam optical tweezers for manipulating superparamagnetic beads.

Applied optics·2021

Same author

Hydroxychloroquine Exhibits a Strong Complex Interaction with DNA: Unraveling the Mechanism of Action.

The journal of physical chemistry letters·2020

Area of Science:

Biophysics
Molecular Biology
Polymer Physics

Background:

The entropic elasticity of DNA is crucial for cellular processes.
Photosensitive drugs like psoralen can intercalate into DNA.
Understanding drug-DNA interactions is vital for therapeutic development.

Purpose of the Study:

To investigate the impact of psoralen on lambda-DNA elasticity.
To determine how different light wavelengths affect psoralen-DNA binding.
To measure the persistence length of DNA-psoralen complexes.

Main Methods:

Single-molecule force spectroscopy using optical tweezers.
Stretching lambda-DNA molecules attached to a microsphere.
Illuminating psoralen-DNA complexes with various light sources.

Main Results:

Psoralen binding significantly alters the elastic properties of DNA.
The wavelength of light influences the psoralen-DNA linkage.
Persistence length measurements quantify changes in DNA elasticity.

Conclusions:

Optical tweezers provide a powerful tool for single-molecule DNA elasticity studies.
Psoralen's interaction with DNA is modulated by light.
This research offers insights into drug-DNA interactions and DNA mechanics.

Related Experiment Videos