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Related Experiment Videos

Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.

Joshua S Waxman1, Anne M Hocking, Cristi L Stoick

  • 1Molecular and Cellular Biology Program, University of Washington School of Medicine, Seattle, WA 98195, USA.

Development (Cambridge, England)
|November 13, 2004
PubMed
Summary

Two zebrafish Dapper (Dpr) proteins, Dpr1 and Dpr2, regulate distinct Wnt signaling pathways. Dpr1 enhances Wnt/beta-catenin activity, while Dpr2 is crucial for Wnt/Ca2+-PCP signaling during embryonic development.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Signaling

Background:

  • Wnt signaling pathways are critical for vertebrate development.
  • Dishevelled (Dvl) proteins are key mediators of Wnt signaling.
  • Dvl-associated proteins, such as Dapper (Dpr), can modulate Wnt pathway responses.

Purpose of the Study:

  • To clone and characterize zebrafish Dpr paralogs, Dpr1 and Dpr2.
  • To investigate the distinct roles of Dpr1 and Dpr2 in Wnt signaling pathways.
  • To determine the functional interactions of Dpr proteins with specific Wnt signaling components.

Main Methods:

  • Cloning and characterization of zebrafish Dpr1 and Dpr2.
  • Loss-of-function studies using zebrafish embryos with hypomorphic Wnt genes.

Related Experiment Videos

  • Gain-of-function experiments to assess the effects of Dpr1 and Dpr2 expression.
  • Analysis of Wnt/beta-catenin and Wnt/Ca2+-PCP signaling activities.

    • Main Results:

    • Endogenous Dpr1, but not Dpr2, enhances Wnt/beta-catenin activity in Wnt8-hypomorphic embryos.
    • Dpr2, but not Dpr1, is essential for convergence extension movements in Stbm or Wnt11-hypomorphic embryos.
    • Dpr1 induces Wnt/beta-catenin target genes and synergizes with Dvl2 and associated kinases.
    • Dpr2 interacts with Wnt/Ca2+-PCP signaling components.

    Conclusions:

    • Zebrafish Dpr1 and Dpr2 are Dvl-associated proteins with distinct functions in Wnt signaling.
    • Dpr1 primarily regulates Wnt/beta-catenin signaling.
    • Dpr2 plays a crucial role in Wnt/Ca2+-PCP signaling, particularly in embryonic morphogenesis.