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Updated: Aug 21, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
Published on: March 30, 2022
Uveal melanocytes produce matrix metalloproteinases-2 and -9 in vitro
Shu-Chen Chu1, Dan-Ning Hu, Shun-Fa Yang
1Department of Food Science, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan.
Abstract:
The purpose of the present study was to investigate the expression of matrix metalloproteinase (MMP)-2 and MMP-9 by cultured human uveal melanocytes, and to test the effects of 12-O-tetradecanoyl-phorbol-13-acetate on the expression of these MMPs. Gelatin zymography of conditioned culture medium from four cultures of human uveal melanocytes (two cultures of iridal melanocytes and two cultures of choroidal melanocytes) detected MMP-2 (72 kDa) and a relatively small amount of MMP-9 (92 kDa), both in the latent form. RT-PCR analysis revealed the MMP-2 mRNA and MMP-9 mRNA in cultured uveal melanocytes. Addition of 12-O-tetradecanoyl-phorbol-13-acetate (10 ng/ml) to the culture medium caused an increase of production of MMP-2 and MMP-9 by cultured uveal melanocytes, and also stimulated the transcription of MMP-2 and MMP-9 of these cells.
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