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Updated: Aug 21, 2026

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Published on: December 12, 2019
Multiple POU-binding motifs, recognized by tissue-specific nuclear factors, are important for Dll1 gene expression in
Kohzo Nakayama1, Kazuko Nagase, Yuriko Tokutake
1Department of Anatomy, Shinshu University, School of Medicine, Matsumoto, Nagano 390-8621, Japan. kohzona@gipac.shinshu-u.ac.jp
Abstract:
We cloned the 5'-flanking region of the mouse homolog of the Delta gene (Dll1) and demonstrated that the sequence between nucleotide position -514 and -484 in the 5'-flanking region of Dll1 played a critical role in the regulation of its tissue-specific expression in neural stem cells (NSCs). Further, we showed that multiple POU-binding motifs, located within this short sequence of 30bp, were essential for transcriptional activation of Dll1 and also that multiple tissue-specific nuclear factors recognized these POU-binding motifs in various combinations through differentiation of NSCs. Thus, POU-binding factors may play an important role in Dll1 expression in developing NSCs.
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