Related Experiment Video

Updated: Aug 21, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Repression of transcription at the human T-cell receptor Vbeta2.2 segment is mediated by a MAX/MAD/mSin3 complex

Marie-Pierre Font1, Myriam Cubizolles, Hervé Dombret

  • 1Unité INSERM 462, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75010 Paris, France.

Biochemical and Biophysical Research Communications
|November 16, 2004
PubMed

Insights

Researchers identified protein complexes regulating cell growth, specifically MAX/MAD family members, mSin3, and N-CoR, binding to a DNA silencer. This binding is dependent on histone deacetylase (HDAC) activity.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cellular Regulation

Background:

  • Identifying protein regulators in complex cellular networks is challenging.
  • Understanding cell proliferation versus differentiation is crucial for development and disease.

Purpose of the Study:

  • To identify protein components of co-regulatory networks involved in cell growth.
  • To investigate the role of histone deacetylases in transcriptional regulation of the T-cell receptor Vbeta2.2 segment.

Main Methods:

  • Surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS)
  • Surface plasmon resonance coupled to electrospray mass spectrometry (SPR-ESI-MS)
  • Immunoelectromobility shift assays (IEMSA)

Main Results:

  • Identified MAX/MAD family members binding to a specific DNA silencer fragment.
  • Identified cofactors mSin3 and N-CoR, known histone deacetylase (HDAC) interactors.
  • Inhibition of deacetylase activity prevented transcription inhibitor complex formation.

Conclusions:

  • The formation of transcription inhibitor complexes at the Vbeta2.2 segment is dependent on HDAC activity.
  • This study elucidates key protein interactions in cell growth regulation.

More Related Videos

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Related Concept Videos

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...