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Updated: Aug 21, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
The copper(II) binding properties of the cyclic peptide c(HGHK)
Justyna Brasun1, Chiara Gabbiani, Mauro Ginanneschi
1Department of Basic Medical Sciences, Wrocław Medical University, Kochanowskiego 14, 51-601 Wrocław, Poland. jbrasun@basmed.am.wroc.pl
Abstract:
The new cyclic tetrapeptide c(HGHK) was synthesised in the solid phase and its complexes with copper(II) were studied in aqueous solution at various pH values by means of potentiometric and spectroscopic methods (UV, EPR, CD). Six mononuclear coordination species were clearly identified within the pH range 3-11. Spectroscopic data strongly suggest sequential formation of N, 2N, 3N and 4N equatorial donor sets around the copper(II) centre from the lowest to the highest pH, involving both imidazole nitrogens and amide nitrogens. A detailed comparison with the copper(II) binding properties of HGHG and Ac-HGHG ligands is also reported.
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