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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Detection of noroviruses in raspberries associated with a gastroenteritis outbreak
Françoise S Le Guyader1, Christian Mittelholzer, Larissa Haugarreau
1Laboratoire de Microbiologie, IFREMER, BP 21105, Nantes cedex 03 44311, France. sleyguyad@ifremer.fr
Abstract:
Following an acute foodborne gastroenteritis outbreak in southern Sweden, stool specimens from five of nine ill patients were found positive for norovirus using reverse transcriptase polymerase chain reaction. Epidemiological data pointed to raspberry cakes as the source of the outbreak. Using a combination of generic and patient-specific primers and novel food analysis methodology (with extraction efficiency control and inhibitor removal), norovirus strains from two different genogroups were directly identified in the contaminated raspberries.
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