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Related Experiment Videos

Microsatellite mutation directed by an external stimulus.

Adele L Schmidt1, Vivek Mitter

  • 1CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Qld 4067, Australia. adele.schmidt@csiro.au

Mutation Research
|November 16, 2004
PubMed
Summary
This summary is machine-generated.

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Fusarium head blight (FHB) infection can induce microsatellite mutations in wheat DNA. This study found that FHB pathogen exposure caused specific DNA changes in affected plant tissues, suggesting an external cue directs mutation.

Area of Science:

  • Genetics
  • Plant Pathology
  • Molecular Biology

Background:

  • Microsatellites are repetitive DNA sequences.
  • The potential for external cues to direct microsatellite mutation is largely unexplored.
  • Fusarium head blight (FHB) is a significant pathogen affecting wheat.

Purpose of the Study:

  • To investigate whether exposure to Fusarium graminearum can induce microsatellite mutations in wheat.
  • To determine if specific microsatellite loci are susceptible to environmentally induced mutation.
  • To characterize the nature of any induced mutations.

Main Methods:

  • Wheat varieties (resistant and susceptible) were grown under controlled conditions.
  • Plants were genotyped at microsatellite loci before and after inoculation with Fusarium graminearum.

Related Experiment Videos

  • Affected tissues were analyzed for mutations using PCR, cloning, and sequencing.

    • Main Results:

    • 58% of inoculated plants acquired a novel allele at the Xgwm112.1 locus within a month.
    • This mutation, a contraction of a (CT)(n) repeat, was only detected in FHB-affected tissue.
    • No mutations were observed in control plants or at other control loci.

    Conclusions:

    • Microsatellite mutation can be induced by external cues, specifically FHB infection.
    • The mutation observed at Xgwm112.1 appears to be a direct response to FHB pathogen exposure.
    • This finding opens new avenues for understanding genome plasticity in response to environmental stress.