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Updated: Aug 21, 2026

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
Published on: May 15, 2014
A natural vaccinia virus promoter with exceptional capacity to direct protein synthesis
Xu Liu1, Marcia Kremer, Steven S Broyles
1Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, IN 47907-1153, USA.
Abstract:
A survey of vaccinia virus promoters, through a reporter gene approach, has identified the viral I1L promoter as having exceptional activity. The I1L promoter exhibited over 10 times the activity of other vaccinia promoters and even rivaled the activity of the bacteriophage T7 promoter in the hybrid vaccinia/T7 expression system. The I1L promoter had high activity in both transient transfection experiments and in the context of recombinant viruses. The I1L promoter should be useful for high-level protein synthesis and poxvirus studies in general.
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