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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Interesterification activity of Rhizopus delemar lipase in phospholipid microemulsions
Takayoshi Komatsu1, Kazuhito Nagayama, Masanao Imai
1Department of Materials Science and Engineering, Kochi National College of Technology, 200-1 Monobe, Nankoku, Kochi 783-8508, Japan.
Abstract:
The interesterification of olive oil with palmitic acid catalyzed by Rhizopus delemar lipase was investigated in phospholipid microemulsion systems. Soybean lecithin was used as the amphiphilic component. The maximal reaction rate was obtained at a buffer pH of 5.5-6.0. The reaction rate was also dependent on the W(L) (= [H2O]/[lecithin]) value and attained a maximum at W(L)=5. The reaction rate reached a maximum at a palmitic acid concentration of 350 mM. The molar fraction of the interesterified product 1,3-dipalmitoyl-3-oleoyl glycerol (POP) in the olive oil was enhanced from 2.8 to 65.6 mol% after 24 h of the reaction.
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