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Modeling and Evaluation of Murine Diabetic Cardiomyopathy Model
Published on: November 29, 2024
Characterization of diabetes-related traits in MSM and JF1 mice on high-fat diet
Misato Kobayashi1, Tamio Ohno, Takeshi Tsuchiya
1Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Abstract:
We examined the effect of a high-fat diet on the diabetes-related traits of the Japanese Fancy mouse 1 (JF1), MSM, and C57BL/6J (B6J) mice. MSM and JF1 mice were derived from Mus musculus molossinus. B6J is a commonly used laboratory strain, with the vast majority of genome segments derived from Mus musculus domesticus and Mus musculus musculus, and is susceptible to high-fat diet-induced type 2 diabetes. None of the strains showed symptoms of diabetes or obesity when fed a laboratory chow diet. Under a high-fat diet, JF1 mice developed impaired glucose tolerance, hyperglycemia, hyperinsulinemia, and obesity. B6J mice fed a high-fat diet mildly developed these diabetes-related traits compared to JF1 mice fed a high-fat diet. JF1 mice fed a high-fat diet were classified as having type 2 diabetes and were susceptible to high-fat diet-induced diabetes and obesity. On the other hand, MSM mice were resistant to high-fat diet-induced diabetes. These results indicate that the JF1 strain, with its unique genetic origin, is a useful new animal model of high-fat diet-induced diabetes and obesity. Further investigations using JF1 mice will help to clarify the role of the high-fat diet on human diabetes and obesity.
Insights
Japanese Fancy (JF1) mice are susceptible to high-fat diet-induced type 2 diabetes and obesity, unlike resistant MSM mice. This makes JF1 mice a valuable new model for studying diet-related metabolic diseases.
Area of Science:
- Metabolic disease research
- Animal models of diabetes
- Dietary impact on health
Background:
- High-fat diets (HFD) are linked to obesity and type 2 diabetes (T2D).
- Understanding genetic susceptibility to diet-induced metabolic diseases is crucial.
- Japanese Fancy (JF1) and MSM mice, derived from Mus musculus molossinus, offer unique genetic backgrounds.
Purpose of the Study:
- To investigate the impact of a high-fat diet on diabetes-related traits in JF1, MSM, and C57BL/6J (B6J) mouse strains.
- To identify novel mouse models for high-fat diet-induced metabolic disorders.
- To compare the susceptibility of different mouse strains to diet-induced diabetes and obesity.
Main Methods:
- Feeding three mouse strains (JF1, MSM, B6J) a standard chow diet and subsequently a high-fat diet.
- Monitoring and comparing diabetes-related indicators such as glucose tolerance, blood glucose levels, insulin levels, and body weight.
- Classifying the development and severity of metabolic disease symptoms across strains.
Main Results:
- JF1 mice fed a high-fat diet exhibited impaired glucose tolerance, hyperglycemia, hyperinsulinemia, and obesity, meeting criteria for type 2 diabetes.
- B6J mice showed milder, but still present, diabetes-related traits under a high-fat diet compared to JF1 mice.
- MSM mice demonstrated resistance to high-fat diet-induced diabetes and obesity.
Conclusions:
- The JF1 mouse strain is a valuable and novel model for studying high-fat diet-induced type 2 diabetes and obesity due to its unique genetic makeup.
- JF1 mice provide a new platform for further research into the mechanisms underlying diet-related metabolic diseases.
- These findings highlight the importance of genetic background in determining susceptibility to diet-induced metabolic disorders, with implications for understanding human disease.
