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Production of L-asparaginase by filamentous fungi
Maria Inez de Moura Sarquis1, Edna Maria Morais Oliveira, Alberdan Silva Santos
1Laboratório de Coleção de Fungos, Departamento de Micologia, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. isarquis@ioc.fiocruz.br
Abstract:
L-asparaginase production was investigated in the filamentous fungi Aspergillus tamarii and Aspergillus terreus. The fungi were cultivated in medium containing different nitrogen sources. A. terreus showed the highest L-asparaginase (activity) production level (58 U/L) when cultivated in a 2% proline medium. Both fungi presented the lowest level of L-asparaginase production in the presence of glutamine and urea as nitrogen sources. These results suggest that L-asparaginase production by of filamentous fungi is under nitrogen regulation.
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