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HPLC profiling and quantification of active principles in leaves of Hedera helix L
B Demirci1, M Goppel, F Demirci
1Department of Pharmaceutical Biology, Faculty of Chemistry and Pharmacy, University of Regensburg, Regensburg, Germany.
Abstract:
Ivy (Hedera helix L., Araliaceae), is an evergreen medicinal and ornamental plant. Depending on leaf polymorphism different shaped ivy leaves were extracted and subsequently analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Quantitative determination of its most prominent saponins hederacoside C (1) and alpha-hederin (2) from different ivy leaf extracts were detected, validated and optimized for quick profiling. The linearity of response, repeatability and reproducibility of the applied RP-HPLC method are reported.
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